2017 Fiscal Year Final Research Report
Genome-wide epigenetic analysis of cloned cattle from the same donor cells
| Project/Area Number |
15K07764
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Kaneda Masahiro 東京農工大学, (連合)農学研究科(研究院), 准教授 (80469840)
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| Research Collaborator |
KIEFER Helene
JOUNEAU Luc
JAMMES Hélène
BALZERGUE Sandrine
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 体細胞クローン牛 / エピジェネティクス / DNAメチル化 / MeDIP-chip |
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| Outline of Final Research Achievements |
Genome-wide epigenetic analysis focusing on DNA methylation levels were conducted using tissues from cloned cattle derived from the same donor cells. Five cloned cattle from the same donor cells and five non-cloned cattle were used for the analysis. Genomic DNA was extracted from liver and MeDIP (Methylated DNA Immunoprecipitation)-chip method was used for DNA methylation analysis. The correlation clustering showed that the difference among individual cattle was larger in non-cloned group compared to cloned group.
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| Free Research Field |
繁殖生物学
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