Functional analyses on a new molecular system, EphA/ephrin-A, in transendothelial migration, infiltration and/or tissue lodgement of monocytes/macrophages

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07769
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Ogawa Kazushige 大阪府立大学, 生命環境科学研究科, 教授 (60231221)
• Research Collaborator: PASQUALE ELENA Sanford Burnham Prebys Medical Discovery Institute, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: EphA / ephrin-A / monocytes / macrophages

Outline of Final Research Achievements

The author found out functions of EphA receptors and ephrin-A ligands related to transendothelial migration, infiltration and/or tissue lodgement of monocytes/macrophages as follows. (1) Expressions of some EphA and ephrin-A members were upregulated/induced during monocyte maturation; Stimulation by EphA as well as ephrin-A, promoted adhesion to an integrin ligand coated surface and formation of protrusions in monocytes. (2) Following ephrin-A1 stimulation, endogenous EphA2 promotes cell adhesion through interaction with integrins and integrin ligands such as ICAM1 and that truncated EphA2 lacking the kinase domain potentiates the adhesion and becomes associated with the integrin/integrin ligand complex in monocytes/macrophages. (3) Truncated EphA2 likely potentiates cell adhesion via integrins as well as infiltration and/or lodgment of a monocyte/macrophage cell line in the red pulp and marginal zone of the mouse spleen, where ephrin-A1 is prominently expressed in the vasculature.

Free Research Field

細胞生物学