Nucleroprin reporter mice with the genome editing technology for the nuclear pore complex research

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07780
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Okayama University
• Principal Investigator: Akiyama Kouyou 岡山大学, 自然生命科学研究支援センター, 特任助教 (20515142)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 実験動物学 / 遺伝学 / 発生工学

Outline of Final Research Achievements

The nuclear pore complex has an important role in various biological natures, such as nuclear form maintenance, the nucleo-cytoplasmic transport and nuclear morphology assembly, but it is suggested that the function of the nuclear pore complex is not necessarily limited to them. It is strongly suggested that Tmem48 null mice phenotypes. Tmem48 is one of nucleoporins, which are the constituent building blocks of the nuclear pore complex. Tmem48 null mice show abnormalities of both the axial skeleton and gametogenesis, although Tmem48 is ubiquitous expression. In this study, it is proposed to elucidate the role of the nucleoporin in vivo by nucleroprin reporter mice, which expressed the fusion gene of Tmem48 and an antibody tag protein or a fluorescence protein by using the genome editing technique, such as CRISPR/Cas9 system.

Free Research Field

実験動物学