Elucidation of immune function through antigen-specific monoclonal T cell receptor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07787
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: University of Yamanashi (2016-2017); Tokyo Metropolitan Institute of Medical Science (2015)
• Principal Investigator: KAMINUMA Osamu 山梨大学, 大学院総合研究部, 准教授 (80342921)
• Co-Investigator(Kenkyū-buntansha): 井上 貴美子 国立研究開発法人理化学研究所, 遺伝子工学基盤技術室, 専任研究員 (70360500)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: クローン動物 / T細胞 / 核移植 / T細胞受容体

Outline of Final Research Achievements

By comparing cloned mice derived from antigen-specific T cells generated by applicants for the first time and mouse lines developed by crossbreeding of the cloned mice with inbred mice and the same and other cloned mice, we clarified the importance of T cell receptor (TCR) to thymic T cell development and T cell subset differentiation. Antigen-induced T cell responses in T cell cloned mice were stronger than those of corresponding TCR-transgenic mice. Furthermore, the in vivo antigen responses in mice expressing either alpha- or beta-chain of TCR derived from cloned mice were stronger than those of inbred mice. The usefulness of antigen specific T cell cloned mice for investigating immune systems and developing immune disease models.

Free Research Field

実験動物学