2017 Fiscal Year Final Research Report
Analysis of insect solute transporters in the liposome system with various phospholipids
Project/Area Number |
15K07795
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
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Research Institution | Tottori University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
泉 洋平 島根大学, 生物資源科学部, 准教授 (10457210)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | アクアポリン / グリセロール / 昆虫休眠 / 低温耐性 |
Outline of Final Research Achievements |
Insects are able to survive adverse climatic conditions. Glycerol and water redistributions in tissues and cells play a key factor under low temperature for cold and/or freeze tolerance. We have explored the membrane proteins of aquaporin (water channel) to clarify the molecular basis for glycerol accumulation in an overwintering insect (the rice stem borer, Chilo suppressalis). The results are as follows: (1) There have several AQP members in the Chilo larvae like the silkworm AQPs. (2) At the trachea, an insect respiratory tissue, we idenfified one water-specific AQP, which is predominantly expressed in the hindgut of silkworms. (3) The expression of this type increased significantly towards winter. (4) Another AQP, a glycerol transporter-type, was found in the midgut and Malpighian tubules. There appears to exist two different types of GLP subfamily in Chilo larvae. (5) An in vitro liposome system failed to construct with these characterized AQPs, that is still underway.
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Free Research Field |
昆虫科学
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