2017 Fiscal Year Final Research Report
Development of optically active lactic acid producing system from non-sterilized by-product of the rice as single raw material using the environment-conscious bioprocess.
| Project/Area Number |
15K07809
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental agriculture(including landscape science)
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
SASANO Kazuo
KASHIWAMURA Takashi
SHIONO Tadahiko
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 光学活性乳酸 / 米加工副産物 / 菌体外プロテアーゼ / T-RFLP法 |
|---|
| Outline of Final Research Achievements |
In this study, we found that extracellular protease activity of a high lactic acid producing bacterium Lactobacillus rhamnosus M-23 was relatively higher than the other isolated LABs from a rice washing drainage storage tank. The results of the T-RFLP analysis showed that SSF culture inoculated with strain M-23 was dominated by Clostridium sp. cluster IX and XIVa at an early stage of SSF culture, respectively. In comparison with an inoculation of relatively low lactic acid producing strain, Clostridium sp. cluster IX mainly dominated with the high accumulation of VFAs (acetic and propionic acid) and the decrease of produced lactic acid in SSF culture compared with the case of strain M-23 inoculation. These results demonstrated the synergetic effect of proteinaceous material removal of the starch particle surface by extracellular protease as well as a repressive effect of dominance formation of Clostridium sp. cluster IX in SSF culture.
|
| Free Research Field |
バイオマス資源学
|
