Development of fluorinated fluorescent probes for membrane protein reconstitution matrices

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07883
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Takagi Toshiyuki 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (10248065)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: フッ素 / 蛍光色素 / 人工脂質 / 二分子膜 / 膜タンパク質

Outline of Final Research Achievements

Organofluorine compounds have considerable much attention in the field of medicinal and agricultural chemistry from the view of point of their unique characteristics such as thermal and chemical stability, surface tension lowering ability, hydrophobicity and lipophobicity. We are engaged in developing partially fluorinated phospholipid membranes as membrane protein reconstitution matrices toward ultra-thin film typed biosensors. As an extension of these researches, we prepared new fluorinated fluorescent probes, and found that the lateral diffusivity in the fluorinated phospholipids bilayers observed with the probes was enough to exhibit suitable fluidity for membrane protein reconstitutions.

Free Research Field

有機フッ素化学

Academic Significance and Societal Importance of the Research Achievements

細胞膜や細胞内膜系に存在する膜タンパク質は、膜を隔てた物質輸送やエネルギー生産・ 変換等の分子認識機能以外に高次の機能も有しており、創薬分野における標的タンパク質と してのみならず、バイオリアクタ等バイオデバイスとしての産業利用が広く検討されている。膜タンパク質の利用を図るには、その機能を低下させることなく化学的・物理的に安定な疎水性脂質環境が必要であり、高疎水・疎油性である部分フッ素化脂質の有用性を評価する技術のひとつを提案した。