2017 Fiscal Year Final Research Report
Elucidation of the splicing regulatory function of an intrinsically disordered protein PQBP1
| Project/Area Number |
15K07886
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
帯田 孝之 富山大学, 大学院医学薬学研究部(薬学), 准教授 (30578696)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | タンパク質 / 天然変性タンパク質 / 相互作用 / アロステリック効果 |
|---|
| Outline of Final Research Achievements |
PQBP1 is composed of a small folded WW domain and a large disordered region. PQBP1 binds to a splicing factor WBP11 and a spliceosomal protein U5-15kD. The hydrophobic area of U5-15kD interacts with the YxxPxxVL motif located in the C-terminal region of PQBP1. In this study, we have shown that the interaction between PQBP1 and U5-15kD is attenuated by the binding of the N-terminal WW domain of PQBP1 to WBP11. Our results also showed that the interaction between U5-15kD and U5-52K strengthen the interaction between U5-15kD and PQBP1.
|
| Free Research Field |
タンパク質科学
|
