Synthesis and characterization of functional hydrogel thin films for cell culture application

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07893
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: Sasai Yasushi 岐阜薬科大学, 薬学部, 准教授 (60336633)
• Co-Investigator(Kenkyū-buntansha): 山内 行玄 松山大学, 薬学部, 准教授 (10461378)
• Co-Investigator(Renkei-kenkyūsha): KONDO Shin-ichi 岐阜薬科大学, 薬学部, 教授 (90240944)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 高分子ブラシ / プラズマ化学 / 細胞培養 / 表面化学

Outline of Final Research Achievements

Highly hydrophilic and non-cell adhesive polymer brush layer was fabricated on a commercial polystyrene dish by surface-initiated atom transfer radical polymerization of sulfobetaine- and phosphobetaine-type zwitterionic methacrylic monomers. Plasma surface treatment was effective to change surface properties of the polymer brushes as the results of plasma-induced reactions. ESR analysis suggested that tertiary end-chain radicals generated by polymer main-chain scission were mainly observed in polymer brushes by plasma-irradiation. In addition, XPS analysis indicated that plasma-irradiation caused rapid de-esterification of methacrylic polymer brushes. By patterning plasma-exposed area on non-cell adhesive zwitterionic methacrylic polymer brushes using a masking sheet, the specific areas which promote the formation of size-controlled spheroid-like cell aggregates were successfully prepared.

Free Research Field

薬品物理化学、高分子化学