Functional analysis of RABL2-CEP19 complex involved in primary cilium formation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07929
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyoto University
• Principal Investigator: Katoh Yohei 京都大学, 薬学研究科, 助教 (90568172)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 一次繊毛 / 鞭毛 / 繊毛病 / 低分子量GTPase / IFT複合体 / 基底小体 / 中心体 / VIPアッセイ

Outline of Final Research Achievements

Mutation of RABL2 and CEP19 genes are known to cause male infertility and morbid obesity, but its molecular mechanism is unknown. In this study, I studied the function of RABL2 and CEP19 involved in primary cilium formation. As a result, I found that RABL2 forms a complex with CEP19 and localizes at the basal body of primary cilia. When RABL2 is converted to GTP-bound form by guanine nucleotide exchange factor, it dissociates from CEP19 and transiently interacts with IFT-B complex via IFT74-IFT81 dimer. These results indicate that RABL2 regulates the entry of the IFT complex into primary cilium.

Free Research Field

分子細胞生物学