Chemical biology analysis of type III secretion system inhibitors and discovery of novel natural ligands

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Kitasato University
• Principal Investigator: Asami Yukihiro 北里大学, 感染制御科学府, 特任講師 (70391844)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Ⅲ型分泌装置 / 天然物化学

Outline of Final Research Achievements

We have identified proteins that selectively bind a T3SS inhibitor, including its known ligand EF-Tu (elongation factor thermo unstable) and protein X encoded by gene X. For this target molecule, three strains were created: a gene X knockout strain, a vector complementary strain, and a gene X complementary strain. Inhibition of hemolysis and secretion of Esp (enterococcal surface protein) family proteins, which are components of the T3SS, were measured in these strains. As a result, hemolysis was found to be inactivated in the gene X knockout strain. This inactivation was partially recovered in the gene X complementary strain. Furthermore, secretion of Esp A, B, C, and D were decreased in the gene X knockout strain. The decrease in secretory levels was partially recovered in the gene X complementary strain. These results indicate that gene X is involved in T3SS expression and also regulates hemolytic activity.

Free Research Field

天然物化学