STUDY ON THE MECHANISMS UNDERLYING INDUCTION OF VBNC STATE AND RE-SUSCITATION FROM VBNC TO GROWING STATE USING PATHOGENIC SALMONELLA

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Osaka University of Pharmaceutical Sciences
• Principal Investigator: Amano Fumio 大阪薬科大学, 薬学部, 教授(移行) (90142132)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: VBNC状態 / サルモネラ / 乾燥耐性 / 抗体産生誘導 / ストレス応答 / 再活性化 / ピルビン酸 / オキサロ酢酸

Outline of Final Research Achievements

The mechanisms underlying induction of VBNC by some kinds of stresses and those concerning resuscitation from the VBNC state to colony-forming state during incubation were studied using pathogenic Salmonella in vitro. Extensive studies were made especially on the changes in the surface antigens of the VBNC, however, they did not succeed in producing adequate results for the further analyses. Finally, we focused on the resuscitation processes of the VBNC Salmonella that had been extremely elevated by addition of pyruvate. In this study, we found that oxaloacetate showed higher resuscitation effects than pyruvate, and other substrates that compose TCA cycle. These results suggest that resuscitation is the necessary condition for characterization of VBNC, and even suitable for definition of VBNC state of bacteria.

Free Research Field

医歯薬学