2017 Fiscal Year Final Research Report
Analysis of segment-specific cadmium transport mechanism in renal proximal tubules
| Project/Area Number |
15K08054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 腎臓 / カドミウム / 輸送 / 近位尿細管 / 金属 / 毒性 |
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| Outline of Final Research Achievements |
To analyze the proximal tubular dysfunction mechanism by cadmium (Cd), we utilized the mouse immortalized proximal tubule cells derived from S1, S2, S3 segments to examine the Cd transport and toxicity. There is no difference in the sensitivity to Cd in S1, S2, S3 cells, we found that the high uptake from the apical side of S3 cells where the expression of ZIP8, the transporter having a high affinity for Cd, is high. Moreover, the induction of Kim-1 which is a sensitive renal failure marker was observed in S1, S2, and S3 cells exposed to low concentrations of Cd. Since the purification of mouse MT protein has been made, it is expected to analyze the transport and toxicity mechanisms of Cd-MT in the future.
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| Free Research Field |
分子環境毒性学
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