Degradation of glycosaminoglycans in cerebral infarction

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Chiba University
• Principal Investigator: Higashi Kyohei 千葉大学, 大学院薬学研究院, 助教 (10463829)
• Co-Investigator(Renkei-kenkyūsha): Toida Toshihiko 千葉大学, 大学院薬学研究院, 教授 (60163945) ; IGARASHI Kazuei 千葉大学, 大学院薬学研究院, 名誉教授研究 (60089597) ; FURIHATA Tomomi 千葉大学, 大学院医学研究院, 講師 (80401008)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 糖衣 / ヘパラン硫酸 / コンドロイチン硫酸 / 脳梗塞 / 酸化ストレス / アクロレイン / ヘパラナーゼ / ヒアルロニダーゼ

Outline of Final Research Achievements

In this study, we observed that heparan sulfate (HS) and chondroitin sulfate (CS) were degraded in ischemic brain lesion. In addition, increased level of heparanase 1 (HPSE1), hyaluronidase (HYAL1) and HYAL4 were observed. When low molecular weight of heparin and CS together with N-acetylcysteine (NAC), a free radical scavenger were administrated to photochemically induced thrombosis model mice, infarct area was decreased compared with NAC only group. These results suggest that not only oxidative stress but also degradation of HS and CS in glycocalyx of endothelial cells are important for the ischemic brain infarction.

Free Research Field

病態分析化学