Experimental investigation regarding the anatomical condition of abnormal cerebrospinal fluid leakage at the lumbosacral spine in cerebrospinal fluid hypovolemia

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08137
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Oita University
• Principal Investigator: MIURA MASAHIRO 大分大学, 医学部, 講師 (50199957)
• Co-Investigator(Kenkyū-buntansha): 内野 哲哉 大分大学, 医学部, 助教 (70423697)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳脊髄液 / 脳脊髄液減少症 / 髄液異常漏出 / 蛍光イメージング / RI脳槽シンチグラフィー / indocyanine green / RIオーロラサイン / 髄膜リンパ管前通液路

Outline of Final Research Achievements

In this study, we used the fluorescent observation of the absorption dynamics of indocyanine green (ICG) to analyze the consistency of positive radioisotope (RI) scintigraphy findings as an indicator for the identification of cerebrospinal fluid hypovolemia (CFH) and dynamics of the ICG in the meninges and within nerve roots via pre-lymphatic channels. In the injection experiment, ICG infiltration was observed bilaterally at all spinal segments in cadavers and Japanese macaques. A bilateral difference in the region of ICG infiltration within the meninges were also found at the search. The macula cribriformis was found at the all nerve root bases using scanning electron microscopy. Our results suggested that positive RI scintigraphy results as a diagnostic standard for CFH not only reflect abnormal leakage of cerebrospinal fluid due to mechanical disruption of the dura mater, but may include many cases of normal cerebrospinal fluid physiologically infiltrating the nerve roots.

Free Research Field

臨床解剖学