2018 Fiscal Year Final Research Report
inhibition of inflammation and neutrophil function by voltage-gated proton channel Hv1/VSOP
| Project/Area Number |
15K08175
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
Okamura Yasushi
Umemoto Eiji
|
|---|
| Project Period (FY) |
2015-04-01 – 2019-03-31
|
| Keywords | プロトンチャネル / 炎症 / 好中球 / 走化性 / ERKシグナル / 活性酸素 |
|---|
| Outline of Final Research Achievements |
Voltage-gated proton channel, Hv1/VSOP, has a role to regulate neutrophil function and inhibit an inflammation in pathogen infection. Hv1/VSOP-deficient mice exhibit excess inflammation in lung after Candida infection. In this study, we found that Hv1/VSOP negatively regulates ROS production in neutrophils stimulated with low concentrations of fMLF. And, we demonstrated that this inhibitory function by Hv1/VSOP in ROS production is necessary for inhibiting ERK-dependent chemotactic response to low fMLF concentrations. These results may suggest that Hv1/VSOP inhibits an inflammation by suppressing neutrophil migration to infection site upon pathogen infection.
|
| Free Research Field |
細胞生理
|
| Academic Significance and Societal Importance of the Research Achievements |
好中球は、感染時にいち早く感染部位に到着して病原菌を除去する役割を担う一方で、過剰な好中球の遊走は炎症を拡大させる負の側面を併せ持つ。そのため、好中球の遊走は適切にコントロールされる必要がある。我々は、電位依存性プロトンチャネルHv1/VSOP1が活性酸素の産生量を抑えることで低濃度の走化性因子に対する走化性応答を抑制することを明らかにした。この結果は、Hv1/VSOPが炎症の早期解決において好中球の遊走能を抑制する可能性を示唆する。今後、このHv1/VSOPの機能に着目した炎症をコントロールする方法の確立につながると期待される。
|
