Roles of fibrinolytic system on vascular permeability after ischemic stroke

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08194
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: Nagai Nobuo 長浜バイオ大学, バイオサイエンス学部, 教授 (90260281)
• Research Collaborator: MATANO Yasuki ; ADACHI Yuta ; KIMURA Nanami
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳梗塞 / 虚血 / 血管透過性 / 血液脳関門 / 線溶系 / プラスミン

Outline of Final Research Achievements

Roles of plasmin activity associated with ischemic stroke was studied using a mouse brain damage model and brain derived endothelial cell line.
It was found that the sequential reduction of the vascular permeable region size was significantly reduced by gene deficient of plasminogen and plasmin activity was distribute apical side of the vessel at the region where vascular permeability was increased. These findings suggested the involvement of plasmin activity on the remodeling of basal lamina. I was also found that the expression of tight junction molecules in cultured brain derived endothelial cell line was suppressed by hypoxia mimicking oxygen-glucose deprivation treatment but not affected by plasmin treatment. These findings suggested plasmin did not contribute to the blood brain barrier disruption under ischemic condition.

Free Research Field

病態生理学