Physiological role of Piezo1 in mechanosensation in keratinocytes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: SUZUKI Yoshiro 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (40348503)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 表皮ケラチノサイト / パッチクランプ / Piezo1 / 機械刺激応答

Outline of Final Research Achievements

Piezo1 is a mechanosensitive Ca2+ permeable cation channel that has been reported to be important for mechanosensation in various tissues in our body. However, the physiological role of Piezo1 in the skin was still unknown. To reveal it, first we tried to identify the localization of Piezo1 in the epidermis and found that Piezo1 was predominantly expressed in keratinocytes. Next, we isolated keratinocytes from the superficial layer of the epidermis and succeeded the patch-clamp recordings. We also breed mice to generate the keratinocyte-specific Piezo1 knockout mice. By combining these, future studies should reveal the functional significance of Piezo1 in the epidermal keratinocytes.

Free Research Field

生理学