2017 Fiscal Year Final Research Report
Molecular mechanism of insulin granules exocytosis via the novel Exophilin-8 complexes.
| Project/Area Number |
15K08295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | インスリン / Rab27 / Exophilin |
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| Outline of Final Research Achievements |
Insulin is produced by beta-cells and is stored as secretory granules in beta-cells of the pancreatic islets. Secretion of insulin requires a series of membrane dynamics, namely, producing granules from the Golgi apparatus, sorting to granules and maturation, and trafficking and fusion to the plasma membrane. Secretory granules transfer to the actin cortex near the plasma membrane before they fuse with plasma membrane. In the present study, we showed that Exophilin-8-knockout mice showed significantly higher blood glucose level, and Exophilin-8-null pancreatic beta-cells exhibited decreased insulin secretion in glucose stimulation and lost insulin granule location at the F-actin rich cell periphery. Furthermore, we found that Exophilin-8 formed the complex with RIM-BP2 and Myosin-VIIa, and the complex had important role for the granule localization to F-actin rich cell periphery.
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| Free Research Field |
細胞生物学、生化学
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