New analysis of triple negative breast cancer by using LentiPlex

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Gunma University
• Principal Investigator: Oyama Tetsunari 群馬大学, 大学院医学系研究科, 教授 (50233622)
• Research Collaborator: YAMANE Arito
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 乳癌 / TNBC / shRNA / CASP-14 / CPA-4

Outline of Final Research Achievements

The short hairpin RNA (shRNA) library is often used to detect promising　candidates for specific molecular target therapies in cancer. For our study, we　first performed a shRNA library analysis to identify cellular viability-related　genes in breast cancer TNBC and non-TNBC cell lines. Then, we performed a transcriptome　analysis ton select highly expressed genes in TNBC compared with non-TNBC cell　lines. Finally, we used transcriptome analysis data from the public database RefEx to select the specific genes not expressed in normal organs.
From these analyses, we identified four genes as candidates for molecular target therapy in TNBC, one is Caspase-14 and the other is Carboxypeptidase A4. We performed several immunohistochemical analysis, using tissue microarray from breast cancer human surgical specimens and migration assay and proliferation assay, using cell lines. We had several significant data.

Free Research Field

人体病理学