Utility of urine cytology as a non-invasive testing in glomerular disease.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Kobe University (2016-2017); Ehime Prefectural University of Health Science (2015)
• Principal Investigator: Ohsaki Hiroyuki 神戸大学, 保健学研究科, 准教授 (80438291)
• Co-Investigator(Renkei-kenkyūsha): SOFUE Tadashi 香川大学, 医学部附属病院, 講師 (80452671) ; KUSHIDA Yoshio 香川大学, 医学部附属病院, 講師 (30325352) ; IRINO Satoshi 愛媛県立医療技術大学, 保健科学部, 講師 (70634418)
• Research Collaborator: TOKUHARA Yasunori 愛媛県立医療技術大学, 保健科学部, 講師 (60746329)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 尿細胞診 / ポドサイト / 糸球体腎炎 / 半月体形成 / WT1

Outline of Final Research Achievements

The purpose of this study was to assess the relationship between urinary WT1-positive cells (podocytes and parietal epithelial cells) and WT1-positive cells in renal biopsy to investigate whether urinary WT1-positive cells are useful for detection of crescent formation. Fifty-two patients with kidney disease were investigated (15 cases with crescentic lesion and 37 cases with non-crescentic lesion) for immunoenzyme staining using anti-WT1 antibody for urine cytology and renal biopsy. Numbers of WT1-positive cells in urine and renal biopsy were counted, respectively. The number of urinary WT1-positive cells in patients with crescentic lesion was significantly higher than in patients with non-crescentic lesion. In addition, the best cut-off value to detect patients with crescentic lesions using urinary was 5 cells/10mL. The results of our study suggest urinary WT1-positive cells can be used to detect patients with crescentic formation using 5 cells/10mL cutoff value.

Free Research Field

人体病理学