2017 Fiscal Year Final Research Report
Molecular mechanism of organ fibrosis and vascular calcification due to metabolic abnormality of phosphate and calcium
| Project/Area Number |
15K08430
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Research Collaborator |
Wei Ran
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 高リン血症 / 血管石灰化 / 酸化ストレス |
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| Outline of Final Research Achievements |
Alteration of phosphate and calcium in the body, especially high phosphate and/or high calcium is closely related to vascular calcification. We investigated the molecular mechanism by which hyperphosphatemia or hypercalcemia causes vascular calcification, focusing on the association between oxidative stress and vascular calcification. We used cultured vascular smooth muscle cells (VSMCs) to examine calcification when they were cultured in the media with high phosphate. When the cultured VSMCs were cultured for 7 days in the medium with high phosphate, calcified materials were precipitated in and out of the cells. At the same time, we found that intracellular oxidative stress signals Keap1/ Nrf2 were altered, indicating that oxidative stress is involved in the mechanism of vascular smooth muscle cell calcification. In addition, we examined the interrelationship between oxidative stress and autophagy, by which gets rid of calcification materials precipitated in the cells.
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| Free Research Field |
病理学
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