The correlation between the structure of PyGM75 and the function in exflagellation of microgamete

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: Tachibana Mayumi 愛媛大学, プロテオサイエンスセンター, 助教 (00301325)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: マラリア / 雄性生殖体 / 鞭毛放出

Outline of Final Research Achievements

PyGM75 expressed on the male gamete surface of Plasmodium yoelii functions in exflagellation of microgametes. In this study, we investigated whether the molecular structure of protease-like domain and transmembrane domain of PyGM75 is involved in PyGM75 function. The transgenic parasite lacking the transmembrane region of pygm75 did not express the PyGM75 on the surface of male gametes. Furthermore, the infectivity to mosquito (the number of oocysts) was decreased compared to the wild type. These results suggested that the transmembrane domain of PyGM75 at C-terminal is important for the expression of PyGM75 on the male gamete and the ability to infect mosquitoes.

Free Research Field

寄生虫学