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2019 Fiscal Year Final Research Report

Virulence analysis of LEE-negative enterohemorrhagic Escherichia coli

Research Project

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Project/Area Number 15K08486
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

IYODA SUNAO  国立感染症研究所, 細菌第一部, 室長 (70300928)

Project Period (FY) 2015-10-21 – 2020-03-31
Keywords腸管出血性大腸菌 / 志賀毒素
Outline of Final Research Achievements

We have isolated several LEE-negative enterohemorrhagic E. coli (EHEC) from patients with severe symptoms, and found that one of them was a "hybrid strain" that are belonging to both EHEC and enteroaggregative E. coli (EAggEC) categories. Using whole genome sequence analysis, we found that this strain possesses a Shiga-toxin 2 converting phage which has highly similar DNA sequences (only few SNPs) to those found in a similar EAggEHEC hybrid strain serotyped O104:H4, cause of massive outbreak resulting several hundreds of HUS cases and more than 50 deaths occurred in Europe, 2011.

Free Research Field

細菌学

Academic Significance and Societal Importance of the Research Achievements

重症例由来の非定型な(LEE非保有型)EHECの解析から、EHECとは別の下痢原性大腸菌カテゴリーである腸管凝集接着性大腸菌(EAggEC)とEHECのハイブリッド株が複数分離されることが判明した。これらのハイブリッド株の一つが保有するStx2ファージの完全長配列を次世代シークエンサーで解析したところ、2011年に欧州各国で大発生したEHECとEAggECのハイブリッド株O104:H4が保有するStx2ファージの配列と数SNPsの違いしかないほぼ同一のファージであることが判明した。本研究の成果は、完全長配列解析によってStxファージの動態を解析し得る可能性を示した点において意義深い。

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Published: 2021-02-19  

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