Investigation of genomic characters specific for high-virulence multidrug-resistant Mycobacterium tuberculosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka Institute of Public Health
• Principal Investigator: Tamaru Aki 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 主幹研究員 (70270767)
• Co-Investigator(Kenkyū-buntansha): 松本 壮吉 新潟大学, 医歯学系, 教授 (30244073) ; 和田 崇之 長崎大学, 熱帯医学研究所, 准教授 (70332450) ; 金子 幸弘 大阪市立大学, 大学院医学研究科, 教授 (90469958)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 多剤耐性結核菌 / SNPs解析 / ゲノム比較

Outline of Final Research Achievements

We found a peculiar genotype, named V02, accounted for 12% of analyzed MDR-Mtb and consisted of only MDR-Mtb from genotyping analysis of Mycobacterium tuberculosis (Mtb). Since the genotype could be considered spreading MDR-Mtb in widely, we identified single nucleotide variants (SNVs) of 15 Mtb isolates belonging V02 genotype by genome wide mapping using Illumina-based short reads. By comparison between the 15 Mtb isolates and outgroup strains, 113 nonsynonymous SNVs were identified as specific to V02 isolates. Four genes with V02 specific SNPs concerned with the biological process of virulence of Mtb. Of 4 genes concerning with virulence, only pknI, reported to regulate the virulence of Mtb, Therefore, the mutation in pknI gene of Mtb belonging V02 genotype might contribute to the high spread of this genotype.

Free Research Field

細菌学