Elucidation of the selective assembly mechanism of influenza virus segmented genome using reconstituted viral ribonucleoprotein complexes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08503
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Kitasato University
• Principal Investigator: MOMOSE Fumitaka 北里大学, 感染制御科学府, 講師 (90332204)
• Research Collaborator: KAWAHARA MADOKA 北里大学, 北里生命科学研究所, 臨時職員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: インフルエンザウイルス / ポリシストロニック発現 / 自己開裂2A配列 / RNP複合体 / パッケージングシグナル / 競合パッケージング / 選択的分節集合

Outline of Final Research Achievements

In order to elucidate the selective assembly mechanism of influenza virus segmented RNA genome-protein complex (vRNP), we attempted to establish an artificial vRNP reconstitution system and to demonstrate the interaction between specific segments by coprecipitation experiments. We also tried to identify the segment assembly/packaging signal sequences present at the termini of each segment by competitive packaging experiments using model virus genome. Since it was suggested as the results that vRNP must be tethered to the membrane surface and laterally diffused in order to selectively assemble, we considered that it is difficult to reproduce such a situation in vitro. It was also suggested that the sequences contributing to the selective assembly or controlling the genome packaging coexist at the termini of viral RNA segment.

Free Research Field

ウイルス学