2017 Fiscal Year Final Research Report
Substrate recognition mechanism of KSHV immune evasion molecule MIR
| Project/Area Number |
15K08504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Showa Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ウイルス / 免疫回避 / 免疫受容体 / 膜タンパク質 / ユビキチンリガーゼ / ナノディスク |
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| Outline of Final Research Achievements |
Kaposi’s sarcoma associated herpesvirus expresses immune evasion molecule family MIR, which is membrane-bound E3 ubiquitin ligases to downregulate several immune proteins on host cell surface. Our previous study revealed that MIR recognizes substrates through transmembrane and extracellular region. In this study, to reveal molecular basis of substrate recognition by MIR, recombinant MIR protein containing two transmembrane regions and extracellular region was prepared and reconstructed in nanodisc, which is artificial lipid bilayer. MIR-nanodisc was subjected to solution NMR analysis; however, HSQC signal was too low to determine structure of MIR. In order to improve molecular stability of MIR, we performed mutational analysis of MIR and revealed that cytoplasmic tail of MIR is essential to stabilize MIR structure.
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| Free Research Field |
タンパク質科学
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