2017 Fiscal Year Final Research Report
Quantitative Analysis of Lipid Species in Chronic Kidney Disease using Imaging Mass Spectrometry
| Project/Area Number |
15K08608
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 非アルコール性脂肪性肝炎 / 慢性腎臓病 / イメージング質量分析 / カルジオリピン |
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| Outline of Final Research Achievements |
Non-alcoholic steatohepatitis (NASH) is drived from metabolic syndrome and simultaneously have a risk to complicate chronic kidney disease. In this study, imaging mass spectrometry (IMS) and conventional liquid-chromatography mass spectrometry (LC/MS) were used for analysis of lipid species on the kidney in NASH model mice. In the results, IMS revealed the specific distribution of molecular species at m/z 1,400-1,500 on the cortex of kidney section in NASH model mice and LC/MS identified these molecular species as cardiolipin with different fatty acids. Cardiolipin is known as a target by oxidative stress because of having four polyunsaturated fatty acids. The comparison with human pathologic specimen about cardiolipn as a molecular marker would be useful for a novel development of treatment and drug.
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| Free Research Field |
分析化学
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