2017 Fiscal Year Final Research Report
Development of immunocomplex pretreatment method for mass spectrometry (MS, Immuno-MS) which is applicable to clinical testing
| Project/Area Number |
15K08611
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 守 千葉大学, 医学部附属病院, 特任准教授 (20401002)
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| Co-Investigator(Renkei-kenkyūsha) |
KODERA Yoshio 北里大学, 理学部, 准教授 (60265733)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 質量分析 / ビタミンD / 細菌同定 |
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| Outline of Final Research Achievements |
Pretreatment methods for mass spectrometry to separate and measure antigen (peptide / protein) from immunocomplex without LC (liquid chromatography) was studied. Specifically, denaturation treatment and ultrafiltration were carried out to separate antigens. Even if MALDI-MS (mass spectrometry by matrix assisted laser desorption / ionization method) ,which is relatively resistant to contaminants in samples is used, it is difficult to obtain sufficient reproducibility for detection data when deviating from the ideal condition.
We further conducted mass spectrometry research on tumor secretion, liver disease marker, vitamin D metabolite etc. and published peer reviewed studies. In MALDI - MS in the bacterial identification test of blood culture specimens, good results of clinical examination level were obtained by pretreatment method not dependent on LC by using special polymer.
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| Free Research Field |
臨床検査
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