2017 Fiscal Year Final Research Report
Establishment of glycosphingolipid analysis in biological samples and application in clinical diagnosis of inherited metabolic disorders
| Project/Area Number |
15K08625
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 糖脂質 / 質量分析 / 先天代謝異常症 |
|---|
| Outline of Final Research Achievements |
Comprehensive quantitative analysis of glycolipids targeting molecular species has not studied fully yet because of the diverse structures of sugar and ceramide moieties of glycosphingolipids(GLS). To establish a method for comprehensive quantitative analysis of GSLs by LC-ESI-MS, the investigation of separation of GSLs using a chiral column has been done. IF-3 chiral column has an advantage in separating GSLs according to not only their ceramide types but also the differences in sugar moieties. Next, the quantitative analysis of GLS in HeLa cells,and also sulfatide standards from porcine brain by multiple reaction monitoring mode. IF-3 was able to detect GSLs present in trace amounts in the total lipid extracts from biological samples.
|
| Free Research Field |
脂質分析化学
|
