Global analysis of cancer-tissue-derived microRNA to improve treatment outcome of cancer of unknown primary

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Tsukuba
• Principal Investigator: OKOSHI Yasushi 筑波大学, 医学医療系, 准教授 (10400673)
• Co-Investigator(Kenkyū-buntansha): 小島 寛 筑波大学, 医学医療系, 教授 (10225435) ; 島居 徹 筑波大学, 医学医療系, 教授 (80235613) ; 玉木 義雄 筑波大学, 医学医療系, 教授 (60188422) ; 沖 明典 筑波大学, 医学医療系, 教授 (60334067) ; 鈴木 久史 筑波大学, 医学医療系, 講師 (40750740)
• Research Collaborator: INADA Katsushige
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 原発不明がん / マイクロRNA / 原発組織 / ホルマリン固定パラフィン包埋 / リンパ節 / 内在性コントロール遺伝子

Outline of Final Research Achievements

To assess the primary tumor of metastatic lesion, we investigated microRNA (miRNA) expression patterns using formalin-fixed, paraffin-embedded (FFPE) lymph node tissues. Initial analysis of 31 samples from 11 types of tumor resulted that the primary site was correctly predicted in 71 %. To achieve higher accuracy, we noticed that an endogenous reference gene for miRNA expression analysis had not been identified in FFPE lymph node with metastatic cancer. A suitable endogenous reference gene should be evaluated to avoid misinterpretation of data and identify true changes in miRNA expression levels. Finally, we found that the combination of three miRNAs, miR-24/miR-103a/let-7a, could be used as a suitable endogenous reference in a lymph node with metastatic cancer.

Free Research Field

医学　血液内科