Novel rapid quantification method of viable bacteria in a septic blood sample can produce an effective biomarker for monitoring patient care

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08642
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Toyama
• Principal Investigator: NIIMI HIDEKI 富山大学, 大学院医学薬学研究部(医学), 准教授 (50401865)
• Co-Investigator(Kenkyū-buntansha): 北島 勲 富山大学, 大学院医学薬学研究部(医学), 教授 (50214797) ; 野手 良剛 富山大学, 附属病院, 主事 (60377364)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 敗血症 / 感染症 / 遺伝子検査 / バイオマーカー / 迅速検査

Outline of Final Research Achievements

Severe sepsis is a common and frequently fetal condition, but current biomarkers do not always reflect the severity of sepsis at a particular point in time. Here we show the development of a novel rapid identification and quantification method of unknown bacteria in a clinical sample using the original technology. We identified and quantified bacteria in septic blood samples, and the quantification results were correlated with the severity of microbial infection . We subsequently examined the time-dependent changes of blood bacterial concentration, and found that the time-dependent changes of blood bacterial concentration were not correlated with those of BT, WBC, CRP, IL-6, and Presepsin. In particular, 24 to 72 hours after antibiotic treatments, blood bacterial concentrations were dramatically decreased. In conclusion, blood bacterial concentration would be useful as a novel biomarker not only to estimate the severity of microbial infection but to monitor the therapeutic effect.

Free Research Field

感染症検査方法の開発