2017 Fiscal Year Final Research Report
Analysis of the regulation by orexins of nociceptive transmission in the spinal dorsal horn by using the patch-clamp technique
| Project/Area Number |
15K08673
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤田 亜美 佐賀大学, 医学部, 准教授 (70336139)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | orexin A / orexin B / excitatory transmission / inhibitory transmission / inward current / spinal dorsal horn / pain / patch clamp |
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| Outline of Final Research Achievements |
Cellular mechanisms underlying the antinociceptive effects of hypothalamic neuropeptides orexins (OXs) in the spinal dorsal horn have not been fully investigated. We examined how OXA and OXB affect spontaneous synaptic transmission in spinal substantia gelatinosa (SG) neurons, which play a pivotal role in regulating nociceptive transmission, by using the patch-clamp technique and adult rat spinal cord slices. As a result, we found out that OXA and OXB concentration-dependently produce a membrane depolarization and/or increased spontaneous L-glutamate release from nerve terminals, both of which actions lead to an increase in neuronal activity of SG neurons, resulting in enhancement by OXA of both glycinergic and GABAergic spontaneous inhibitory transmission and by OXB of only glycinergic transmission; these activities are mediated by orexin-1 and orexin-2 receptor activation, respectively. These actions of OXA and OXB could contribute to their antinociceptive effects.
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| Free Research Field |
神経生理学
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