Establishment of pain evaluation system by using projection neuron-specific calcium imaging in the spinal dorsal horn

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08683
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Kansai Medical University
• Principal Investigator: NISHIDA Kazuhiko 関西医科大学, 医学部, 助教 (80448026)
• Co-Investigator(Kenkyū-buntansha): 松村 伸治 関西医科大学, 医学部, 准教授 (70276393) ; 伊藤 誠二 関西医科大学, 医学部, 教授 (80201325)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脊髄後角 / 疼痛 / 投射ニューロン / カルシウムイメージング

Outline of Final Research Achievements

In order to establish projection neuron-specific calcium imaging in the spinal dorsal horn, I performed birthdate and lineage analyses of these neurons and aimed at the identification of their specific markers. Birthdate analysis using EdU revealed that most projection neurons are born between E9.5 and E10.5 in the mouse. In the developing dorsal neural tube, six classes of neuronal populations (dI1-dI6) are born from distinct progenitor domains around E10. Further analysis using reporter mice labeling Lbx1 lineage neurons (dI4-dI6) and histochemistry demonstrated that most projection neurons are derived from the dI5 progenitor domain.

Free Research Field

神経科学、分子生物学