2017 Fiscal Year Final Research Report
Replication competence and the factors of clinical isolates for prevention of transmission and disease development to AIDS
| Project/Area Number |
15K08734
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Epidemiology and preventive medicine
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| Research Institution | Tottori University |
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Principal Investigator |
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| Research Collaborator |
Kanai Kyosuke
Tsuneki Akeno
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | HIV / 薬剤耐性 / 増殖能 / フィリピン |
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| Outline of Final Research Achievements |
The drug resistance rate was estimated to be 9.2% among the 2,629 referred samples from patients receiving antiretroviral treatment in 2015-2016 in the Philippines. Thirty-seven HIV strains were isolated from 142 HIV-positive serum/plasma samples of patients harboring drug-resistant strains with detectable viral load after a 12-month treatment of drug-naive patients. The replication competence was then assessed by re-inoculation into seronegative peripheral blood mononuclear cells in vitro. The viral yield correlated with the plasma viral load. However, more progeny viruses were produced after the in vitro re-inoculation than the corresponding plasma viral load. Intrinsic replication competence could be assessed by the in vitro re-inoculation system, but not be estimated from in vivo plasma viral load. These results suggest that the replication competence of HIV become an important marker especially when a drug regimen should be altered.
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| Free Research Field |
ウイルス学、国際保健
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