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2017 Fiscal Year Annual Research Report

Exposure to mineral oil at working environment and autoimmunity

Research Project

Project/Area Number 15K08790
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

佐藤 実  産業医科大学, 産業保健学部, 教授 (90162487)

Co-Investigator(Kenkyū-buntansha) 黒田 嘉紀  宮崎大学, 医学部, 教授 (50234620)
保利 一  産業医科大学, 産業保健学部, 教授 (70140902)
田中 晋  産業医科大学, 産業保健学部, 講師 (70280253)
Project Period (FY) 2015-04-01 – 2018-03-31
Keywords自己抗体 / 抗核抗体 / 鉱物油 / 環境因子
Outline of Annual Research Achievements

平成29年度は鉱物油に暴露される作業場の研究参加、試料の収集への交渉を継続し、約200人の鉱物油曝露作業者、コントロールとして鉱物油曝露のない事務系職員の200人をリクルートし、情報、資料を収集した。
また、アレルギー性疾患、自己免疫疾患、自己抗体産生およびその特異性との関連を調べるために血清のTNF-alpha, IL-6, IL-4, IL-5, IL-12, IFN-gammaなどのサイトカインを外注で測定した。
本研究で行う血清の免疫学的検査のうち最も陽性の頻度が高く、解析に有用と考えられる核のdense fine speckled 70 (DFS70)に対する抗体が最重要と考えられたので、 同抗体検出システムの確立に重点を置いた。他のコホートの検査結果から、酵素免疫測定法(ELISA)による抗DFS70抗体の陽性と蛍光抗体法による染色パターンとが一致しない例がある ことが判明したため、アミノ酸349-435のDFS70に特異的で52kDのsplicing variantと重複しない部分のリコンビナント蛋白とアミノ酸1-435の全長リコンビナント蛋白を用いて 酵素免疫測定法(ELISA)でDFS70に対する自己抗体を測定し、その両者間、蛍光抗体法による典型的なdense fine speckledパターン、ウエスタンブロット法によるDFS70蛋白に対する反応、との関連を調べ、何をもって抗DFS70抗体陽性とすべきかについて検討した。これと平行して2種の抗DFS70抗体のELISAで希釈曲線作成に用いる血清のスクリーニングと希釈条件も検討した。
今までに確立された系に 加え、 鉱物油曝露に伴う慢性の肺の炎症でも検出される可能性のある抗Ki/SL/PMSE3, replication protein A (RPA)抗体測定 のためのELISAの条件を検討した。

  • Research Products

    (14 results)

All 2018 2017

All Journal Article (13 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 13 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Detection of anti-mitochondrial antibodies by immunoprecipitation in patients with systemic sclerosis2018

    • Author(s)
      Ceribelli A, Isailovic N, De Santis M, Generali E, Satoh M, Selmi C
    • Journal Title

      J Immunol Methods

      Volume: 452 Pages: 1-5

    • DOI

      10.1016/j.jim.2017.10.001

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Survival of Motor Neuron (SMN) Complex in a patient with necrotizing autoimmune myopathy2018

    • Author(s)
      Amlani A, Hazlewood GS, Hamilton L, Satoh M, Fritzler MJ
    • Journal Title

      Rheumatology

      Volume: 57 Pages: 199-200

    • DOI

      10.1093/rheumatology/kex392

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Immune-mediated thrombocytopenic purpura and hypothyroidism in a lung cancer patient treated with nivolumab2018

    • Author(s)
      Jotatsu T, Oda K, Kawanami T, Kido T, Satoh M, Yatera K
    • Journal Title

      Immunotherapy

      Volume: 10 Pages: 85-91

    • DOI

      10.2217/imt-2017-0100

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies2018

    • Author(s)
      S. Calise J, Zheng B, Hasegawa T, Satoh M, Isailovic N, Ceribelli A, Andrade LEC, Boylan K, Cavazzana I, Fritzler MJ, de la Torre IG, Hiepe F, Kohl K, Selmi C, Shoenfeld Y, Tincani A, Chan EKL, and the IUIS Autoantibody Standardization Committee
    • Journal Title

      Clin Chem Lab Med

      Volume: 印刷中 Pages: -

    • DOI

      10.1515/cclm-2017-1152

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] International Consensus on Antinuclear Antibody Patterns on defining negative results and recommendation in reporting unidentified patterns2018

    • Author(s)
      Herold M, Klotz W, Andrade LEC, Conrad K, Damoiseaux J, Fritzler MJ, von Muhlen, Satoh M, Chan EKL and the other members of the Executive Committee of ICAP
    • Journal Title

      Clin Chem Lab Med

      Volume: 印刷中 Pages: -

    • DOI

      10.1515/cclm-2018-0052

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Elevated α-defensin levels in plasma and bronchoalveolar lavage fluid from patients with myositis-associated interstitial lung diseaseBMC Pulmonary Medicine2018

    • Author(s)
      Sakamoto N, Ishimoto H, Kakugawa T, Satoh M, Hasegawa T, Tanaka S, Hara A, Nakashima S, Yura H, Miyamura T, Koyama H, Morita T, Nakamichi S, Obase Y, Ishimatsu Y, Mukae H
    • Journal Title

      BMC Pulmonary Med

      Volume: 18 Pages: 44

    • DOI

      10.1186/s12890-018-0609-5

    • Peer Reviewed / Open Access
  • [Journal Article] A comprehensive overview on myositis-specific antibodies: New and old biomarkers in idiopathic inflammatory myopathy2017

    • Author(s)
      Satoh M, Tanaka S, Ceriblli A, Chan EKL
    • Journal Title

      Clin Rev Allergy Immunol

      Volume: 52 Pages: 1-19

    • DOI

      10.1007/s12016-015-8510-y.

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Spontaneous improvement of interstitial pneumonia in a patient with autoimmune features2017

    • Author(s)
      Oda K, Kawaguchi T, Satoh M, Yatera K
    • Journal Title

      Internal Med

      Volume: 56 Pages: 1607-1608

    • DOI

      10.2169/internalmedicine.56.8082

    • Peer Reviewed
  • [Journal Article] Myositis-specific autoantibodies and their association with malignancy in Italian patients with polymyositis and dermatomyositis2017

    • Author(s)
      Ceribelli A, Isailovic N, De Santis M, Generali E, Fredi M, Cavazzana I, Franceschini F, Satoh M, Selmi C
    • Journal Title

      Clin Rheumatol

      Volume: 36 Pages: 469-475

    • DOI

      10.1007/s10067-016-3453-0

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Calcinosis in poly-dermatomyositis: clinical and laboratory predictors and treatment options2017

    • Author(s)
      Fredi M, Bartoli F, Cavazzana I, Ceribelli A, Carabellese N, Tincani A, Satoh M, Franceschini F
    • Journal Title

      Clin Exp Rheumatol

      Volume: 35 Pages: 303-308

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] B cell tolerance to deiminated histones in BALB/c, C57BL/6, and autoimmune-prone mouse strains2017

    • Author(s)
      Dwivedi N, Hedberg A, Zheng YY, Neeli I, Satoh M, Morel L, Rekvig OP, Radic M
    • Journal Title

      Frontiers Immunol

      Volume: 8 Pages: 362

    • DOI

      10.3389/fimmu.2017.00362

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy2017

    • Author(s)
      Momohara-Ogawa M, Muro Y, Satoh M, Akiyama M
    • Journal Title

      Clin Chim Acta

      Volume: 471 Pages: 304-307

    • DOI

      10.1016/j.cca.2017.06.022

    • Peer Reviewed
  • [Journal Article] Clinical features in patients with anti-aminoacyl-tRNA synthetase antibody positive idiopathic interstitial pneumonias2017

    • Author(s)
      Yura H, Sakamoto N, Satoh M, Ishimoto H, Hanaka T, Ito C, Hasegawa T, Tanaka S, Miyamura T, Nakashima S, Hara A, Kakugawa T, Oda K, Kido T, Obase Y, Ishimatsu Y, Yatera K, Mukae H
    • Journal Title

      Resp Med

      Volume: 132 Pages: 189-194

    • DOI

      10.1016/j.rmed.2017.10.020

    • Peer Reviewed
  • [Presentation] Autoantibodies in interstitial lung disease and lung cancer2017

    • Author(s)
      Minoru Satoh
    • Organizer
      13th Dresden Symposium on Autoantibodies and Autoimmunity
    • Int'l Joint Research / Invited

URL: 

Published: 2018-12-17  

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