Study on establishment of forensic genetic analysis of degraded DNA samples and improvement of accuracy in the judgment

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08868
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Shinshu University
• Principal Investigator: ASAMURA Hideki 信州大学, 学術研究院医学系, 教授 (80324250)
• Co-Investigator(Kenkyū-buntansha): 佐藤 紀子 信州大学, 学術研究院医学系, 助手 (00649254) ; 塩崎 哲也 信州大学, 医学部, 助教(特定雇用) (00722018) ; 亀井 佐矢子 信州大学, 医学部, 特任助教 (10735153) ; 林 徳多郎 信州大学, 学術研究院医学系, 助教 (50600607) ; 布谷 美弥 信州大学, 医学部, 特任助教 (10759921)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: DNA多型 / INDEL / 劣化DNA

Outline of Final Research Achievements

The objective of this research is to improve accuracy in analyzing degraded DNA samples. We researched Japanese population genetic data of 19 insertion-deletion polymorphisms and 11 X-STRs in X chromosome using newly developed multiplex PCR systems. We investigated suitable parts for DNA analysis from hard tissues and researched the optimal conditions of decalcification in DNA extraction from skeletal remains.

Free Research Field

法医学