Elucidation of intrathrombotic mechanism of autophagic functions in the deep vein thrombosis formation and its application to forensic medicine

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08880
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: Nosaka Mizuho 和歌山県立医科大学, 医学部, 助教 (00244731)
• Co-Investigator(Kenkyū-buntansha): 近藤 稔和 和歌山県立医科大学, 医学部, 教授 (70251923) ; 木村 章彦 和歌山県立医科大学, 医学部, 博士研究員 (60136611) ; 石田 裕子 和歌山県立医科大学, 医学部, 講師 (10364077)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 深部静脈血栓塞栓症 / 血栓陳旧度 / オートファジー / p62 / LC3

Outline of Final Research Achievements

Deep vein thrombosis (DVT) is multifactorial and often results from a combination of risk factors such as obesity, pregnancy, aging and malignancy. Eight-week-old male wild type mice were employed in this study. DVT was induced by the ligation of inferior vena cava. At 1, 3, 5, 7, 10, 14 and 21 days, immunohistochemical analyses were performed. In the murine thrombi, the LC3- or p62-positive cells were detected. The LC3-positive cells were observed all thrombus samples (n=5 per group). But in the thrombus age of 1 day, p62-positive cells were not detected and p62/LC3 ratios were nearly zero. After 3 days, p62-positive cells were detected, and p62/LC3 ratios were over 1.0. At 14 days, there were much p62-positive cells exceeded LC3+ cells, and p62/LC3 ratios reached over 2.0. Our observations indicated the detection of intrathrombotic LC3 and p62 could be a useful marker for thrombus age determination.

Free Research Field

法医学