• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2017 Fiscal Year Final Research Report

The mechanism of metastasis and metastasis suppression in gastric carcinoma by molecular interactions between intracellular target molecules and metastasis suppressor protein Nm23-H1

Research Project

  • PDF
Project/Area Number 15K08951
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionKochi Gakuen College (2016-2017)
Kochi University (2015)

Principal Investigator

MURAKAMI MASANAO  高知学園短期大学, その他部局等, 准教授(移行) (80571017)

Co-Investigator(Kenkyū-buntansha) 大畑 雅典  高知大学, 教育研究部医療学系基礎医学部門, 教授 (50263976)
Research Collaborator Derek Kennedy  グリフィス大学, エスキティス研究所, 准教授
Project Period (FY) 2015-04-01 – 2018-03-31
Keywords胃がん / がん転移抑制 / Nm23-H1 / Asef / G3BP
Outline of Final Research Achievements

We had identified several molecules as a target protein of metastasis suppressor protein Nm23-H1. To clear the mechanism of how cancer cells were obtained ability of migration, we investigated the relationship between Nm23-H1 and candidate of binding proteins which we identified previously. ①Asef2 was identified as a target protein of Nm23-H1, which was stimulated by adenomatous polyposis coli. The GEF activity of Asef was enhanced in scirrhous type gastric carcinoma cell lines and then led to high migration activities, however exogenous Nm23-H1 again suppressed GEF activity. ②G3BP1, 2a and 2b (G3BPs) that are associated with breast cancer and pancreatic cancer, were also bound to Nm23-H1, but there was a difference in the binding strength. Each G3BP molecules have different functions. G3BP1 is known to associate with stress granules formation. Co-transfected cells of G3BPs and Nm23-H1 showed co-localization in the cytoplasm as punctate signals and in all cases.

Free Research Field

腫瘍ウイルス学

URL: 

Published: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi