Development of practical method for the diagnosis of infection and antibiotics susceptibility of H. pylori

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Handa Osamu 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90381970)
• Co-Investigator(Kenkyū-buntansha): 内藤 裕二 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00305575) ; 水島 かつら 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20564115) ; 木下 健司 武庫川女子大学, 薬学部, 教授 (70441219)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 唾液 / ヘリコバクター・ピロリ / PCR / 感染診断

Outline of Final Research Achievements

Diagnosis for H. pylori requires time and expenses. In this study, we focused on "the existence of H. pylori in saliva" as previously reported and by using saliva, attempted to develop a rapid and inexpensive H. pylori infection diagnosis and an antibiotic susceptibility test. First, using a standard solution in which H. pylori was reliably present, PCR was carried out with a template (primer) of H. pylori DNA as previously reported and an H. pylori-specific peak was recognized. However, in patients saliva (infected / non-infected), no specific peak was observed. We tried modification of PCR condition, use of other primers, concentration of specimen, change to primer for toxin DNA of H. pylori, but H. pylori in saliva was not detectable.

Free Research Field

ヘリコバクター・ピロリ、消化器内科学