Analysis of DNA methylation and microRNA expression in hepatocellular carcinoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Yasui Kohichiroh 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30323695)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 肝細胞癌 / マイクロRNA / DNAメチル化

Outline of Final Research Achievements

Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and can act as tumor suppressors or oncogenes. To identify miRNA involved in the development of HCC, a genome-wide miRNA gene expression analysis was performed in paired HCC tumors and non-tumorous liver tissues from patients with primary HCC. We also screened for genes with promoter DNA hypermethylation using a genome-wide DNA methylation microarray analysis in the same samples. We found that miR-214 and miR-199a were significantly down-regulated by aberrant promoter hypermethylation of their host gene, DNM3OS, in HCC tumors compared to the non-tumorous liver tissues.

Free Research Field

肝臓病学、腫瘍学