2017 Fiscal Year Final Research Report
Analysis of DNA methylation and microRNA expression in hepatocellular carcinoma
Project/Area Number |
15K09017
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
Yasui Kohichiroh 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30323695)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Keywords | 肝細胞癌 / マイクロRNA / DNAメチル化 |
Outline of Final Research Achievements |
Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and can act as tumor suppressors or oncogenes. To identify miRNA involved in the development of HCC, a genome-wide miRNA gene expression analysis was performed in paired HCC tumors and non-tumorous liver tissues from patients with primary HCC. We also screened for genes with promoter DNA hypermethylation using a genome-wide DNA methylation microarray analysis in the same samples. We found that miR-214 and miR-199a were significantly down-regulated by aberrant promoter hypermethylation of their host gene, DNM3OS, in HCC tumors compared to the non-tumorous liver tissues.
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Free Research Field |
肝臓病学、腫瘍学
|