Next-generation sequencing of pancreatic juice to diagnose malignant intraductal papillary mucinous tumor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: University of Yamanashi
• Principal Investigator: TAKANO Shinichi 山梨大学, 大学院総合研究部, 講師 (80377506)
• Research Collaborator: ENOMOTO Nobuyuki 山梨大学, 大学院総合研究部, 教授 (20251530)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: IPMN / 膵液 / 次世代シークエンス

Outline of Final Research Achievements

Intraductal papillary mucinous neoplasms (IPMNs) are recognized as one of the risk factors and precursor of pancreatic cancer. To diagnose malignant IPMN before surgery, we conducted gene analysis of resected IPMN by next-generation sequencing and identified TP53 mutation and SMAD4 loss as malignant markers for IPMN. Moreover, we tried to detect these gene alterations with pancreatic juice samples which could be obtained before surgical operation, and we succeeded to detect TP53 mutations in pancreatic juice samples. In the future, we should develop methods to detect these gene alterations with blood samples.

Free Research Field

胆道・膵臓