Functional analysis of Polycomb group proteins in adult heart

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Shirai Manabu 国立研究開発法人国立循環器病研究センター, 創薬オミックス解析センター, 室長 (70294121)
• Co-Investigator(Renkei-kenkyūsha): MORISKI TAKAYUKI 国立循環器病研究センター, 研究所, 部長 (30174410) ; MORISKI HIROKO 国立循環器病研究センター, 研究所, 室長 (40311451)
• Project Period (FY): 2015-10-21 – 2018-03-31
• Keywords: ポリコーム遺伝子 / 心筋細胞 / 恒常性維持

Outline of Final Research Achievements

To understand the molecular mechanisms of Polycomb group (PcG) proteins in maintaining the cardiac functions, we analyzed Pcgf5 (PcG ring finger 5) functions at adult stage. As reported previously, Pcgf5 formed PRC1.5 complex with Ring1b and RYBP in adult mouse left ventricle (LV). We also found casein kinase 2 alpha 1 (CK2α1) and histone deacetylase 2 (HDAC2) associated with Pcgf5 and other PRC1.5 components. In several phosphoproteins, significant alterations were observed in young and aged Pcgf5-deficient LV. We also compared gene expressions in young and aged LV by RNA-seq analysis and defined specific expression changes in aged Pcgf5-deleted LV. These findings revealed that Pcgf5 plays a pivotal role in maintaining the cardiac function in aged mice.

Free Research Field

循環器疾患