2017 Fiscal Year Final Research Report
Pathogeneis of COPD: comparison of SASP in COPD and pulmonary fibrosis
| Project/Area Number |
15K09193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | COPD / 肺線維症 / 細胞老化 / SASP |
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| Outline of Final Research Achievements |
To compare the degree of cellular senescence and senescence-associated secretory phenotype (SASP) in COPD and pulmonary fibrosis, lung tissues samples were subjected to immunostaining for P16 and P21. Double-staining for P16 and phosphorylated NF-κB was also performed to verify SASP. A greater degree of enhancement of P16 and P21 expression was evident in idiopathic pulmonary fibrosis (IPF) and connective tissue disease-associated interstitial lung disease （CTD-ILD）, as compared to COPD and normal lungs. Immunostaining for P16 also revealed enhanced expression of this protein marker in COPD as compared to normal lungs. There were no significant differences in the phosphorylated NF-κB expression rate of P16-positive cells among IPF, CTD-ILD and COPD. These results suggest that cellular senescence is more pronounced, and SASP expression more conspicuous, in interstitial lung diseases, such as IPF, than in COPD.
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| Free Research Field |
呼吸器内科学
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