Identification of molecular mechanisms for AQP2 activation to deveplop its inhibitor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09286
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: YUI Naofumi 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (00633976)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 水チャネル / リン酸化 / 脱リン酸化 / 細胞内輸送 / 集合管 / バソプレシン

Outline of Final Research Achievements

In this study, we developed a phospho-specific AQP2 immuno-isolation technique, and identified that vasopressin-mediated AQP2 Ser-261 phospho-regulation is processing in combination with Ser-256 and Ser-269 phosphorylation. pS256-pS261-AQP2 is phophorylated at Ser-269 to be pS256-pS261-pS269 triplly phospho-form, and then dephosphorylated at Ser-261 to be transformed into pS256-pS269-AQP2. We further revealed that the subsequent Ser-261 dephosphorylation was required to be accumulated in apical plasma membrane in concert with prior Ser-256 and Ser-269 phosphorylation.

Free Research Field

腎臓内科学