Profiling of Matrix Vesicles obtained from cells in various differentiation states.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Kansai University of Health Sciences
• Principal Investigator: Itoh Shunji 関西医療大学, 保健医療学部, 准教授 (50275351)
• Co-Investigator(Kenkyū-buntansha): 鍵弥 朋子 関西医療大学, 保健医療学部, 助教 (50717650) ; 畑村 育次 関西医療大学, 保健医療学部, 教授 (80336883)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 基質小胞 / 異所性石灰化

Outline of Final Research Achievements

Calcification is an essential process for the formation of hard tissues. And ectopic calcification causes many diseases. However, the mechanism of calcification has been not cleared yet. It has been unknown whether the mechanisms of normal and ectopic calcification are identical. To identify molecules constituting the MV and elucidate the calcification mechanism, we studied using ATDC5 cells. The calcification of ATDC5 in the differentiated state is stimulated by the addition of inorganic phosphorus (Pi). Our results showed that the MVs were formed in the undifferentiated state. It was suggested that ATDC5 cells formed both MV without calcification ability and MV with calcification ability, depend on their differentiation state. Moreover, when ATDC5 cells differentiating to the articular cartilage-like cell, MV formation was observed but their calcification was not observed. We compared the MVs of these differentiated states and identified molecules necessary for the calcification.

Free Research Field

分子生物学