2017 Fiscal Year Final Research Report
Association of between oxidative modification of alpha-synuclein under dopamine metabolism and intracellular stability or propagation
| Project/Area Number |
15K09315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | パーキンソン病 / synuclein / 細胞間伝播 / 酸化修飾 |
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| Outline of Final Research Achievements |
The association between oxidative modification of α-synuclein under dopamine metabolism and intracellular stability or propagation of α-synuclein was investigated. α-Synuclein was oxidatively modified at 127th methionine residue under dopamine existing condition and was controlled its stability by autophagy/lysosome system. Secretion of α-synuclein was dependent not only on exosome release but also on non-exosomal release. Secretion of α-synuclein was enhanced under dopamine metabolism. In order to detect oxidative modification of α-synuclein at 127th methionine residue, specific antibody against α-synuclein including methionine sulfoxide was raised. Using this antibody oxidative modification of α-synuclein was successfully detected.
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| Free Research Field |
神経内科学
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