Development of new theraphy of brain protection, dementia prevention and neuroregeneration focusing on astrocytes in cerebral infarction

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Okayama University
• Principal Investigator: Deguchi Kentaro 岡山大学, 医学部, 客員研究員 (80467753)
• Co-Investigator(Kenkyū-buntansha): 太田 康之 岡山大学, 大学病院, 講師 (20746854) ; 菱川 望 岡山大学, 大学病院, 助教 (90378175)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳血管障害 / 低酸素ストレス / 酸化ストレス / アストロサイト

Outline of Final Research Achievements

In this study, we focused on the role of astrocytes in hypoxia and oxidative stress of cerebral infarction, and analyzed it using mouse focal cerebral ischemia model. Immunohistochemistry of cerebral tissue sections showed remarkable increased expression of HIF1α, a hypoxic stress marker, around the cerebral infarction lesions 12 hours after the cerebral infarction, and the highest expression of GSH, antioxidant stress marker, 72 hours after the onset of cerebral infarction. In vivo imaging and on immunohistochemical analyses, the expression of Nrf2, an oxidative stress marker, peaked after 24 hours, and Nrf2 was expressed in both neurons and astrocytes. This study suggested that hypoxic stress was first involved in cerebral infarction, then oxidative stress was involved, in which astrocyte acted mainly.

Free Research Field

医歯薬学