2018 Fiscal Year Final Research Report
Re-evaluation of pathophysiology in diabetes by the receptor-mediated glucagon assay
| Project/Area Number |
15K09373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Research Collaborator |
YANAGIMACHI Tsuyoshi
TAKEDA Yasutaka
HONJO Jun
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Keywords | グルカゴン / 膵島 / 測定法 |
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| Outline of Final Research Achievements |
We established novel methods to measure glucagon, GIP and GLP-1 beaded on cell-based receptor-mediated assays. We strongly speculate that these assays can reflect to physiological actions of those hormones through studies in vitro and in vivo, measuring samples during meal tolerance tests from non-dialect and diabetic subjects.
Furthermore, we established the method for selective collection of proglucagon-positive cells and identified glucagon expression in the gastrointestinal tract other than the pancreas.
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| Free Research Field |
糖尿病学、内分泌学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究においてグルカゴン受容体結合活性に基づき、より生理的なグルカゴン作用を反映するグルカゴン測定系を樹立すること、その測定系を用いて血液や細胞・組織抽出物などを測定すること、さらに既存のイムノアッセイ法と比較検討することは、基礎研究のみならず糖尿病の病態を臨床的に再確認する上で必須であり、かつ学術的に重要で、糖尿病治療へ新たなアプローチへ応用できると確信している。
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