Mechanism based development of differentiation method for pancreatic bud-like cells from human pluripotent stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09385
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Kyoto University
• Principal Investigator: Toyoda Taro 京都大学, iPS細胞研究所, 講師 (60593530)
• Co-Investigator(Renkei-kenkyūsha): OSAFUNE Kenji 京都大学, iPS細胞研究所, 教授 (80502947) ; WATANABE Akira 京都大学, iPS細胞研究所, 特定拠点助教 (60506765)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: NKX6.1 / PDX1 / 膵臓 / iPS細胞 / ES細胞 / 細胞骨格 / 糖尿病

Outline of Final Research Achievements

Pancreatic bud/epithelial cells (NKX6.1+ cells) are considered as pancreas-committed cells. Induction of the NKX6.1+ cell is a critical step for the generation of pancreatic cells from human pluripotent stem cells. However, the induction method and mechanism have not yet been fully established. In this study, we identified low molecular weight compounds which promote differentiation into NKX6.1+ cells from human iPS cells. Based on the analysis of the compounds, we found that cytoskeleton regulating molecules are involved in the part of the mechanisms. Our differentiation protocol established in this study and the mechanism may be useful for both basic research and clinical application using pancreatic cells.

Free Research Field

再生医学